Greenhouse gas emissions and net energy production of dark fermentation from food waste followed by anaerobic digestion

There are diverse estimations regarding the carbon reduction effects of alternative hydrogen production technologies. This study assessed the greenhouse gas (GHG) emissions and energy balance of a two-stage process combining dark fermentative hydrogen production and anaerobic digestion from food waste using the cradle-to-gate life cycle assessment (LCA). The system boundary included collection and transportation, pretreatment and feedstock storage, dark fermentation , H 2 purification, anaerobic digestion and heat and power generation and the estimated GHG emission was compared with a single anaerobic digestion of food waste. GHG emission of the biohydrogen production was estimated as 2.48 kg CO₂-eq per kg H₂ without considering avoided emissions from heat and power generation. The environmental impacts were majorly influenced by electricity use. The net energy ratio of the two-stage process was calculated to be 8.18, confirming a net energy gain and the potential GHG emission avoidance for electricity and heat use. Given that the single-stage anaerobic digestion of 16 tons of food waste is replaced by the two-stage dark fermentation process, 76.6 kg CO₂-eq would be avoided. Sensitivity analysis revealed that energy-saving strategies are the most sensitive factors for achieving positive net energy production and low global warming potential . • Fermentative H 2 from food waste would emit 2.48 kg CO 2-eq /kg H 2 . • The key parameters for greenhouse gas emissions were energy consumption and H 2 yield. • Greenhouse gas was mainly emitted in H 2 purification step. • Combined bio-H 2 and anaerobic digestion showed a high net energy ratio of 8.18