Identification and Growth Characteristics of Cellulase Producing Bacteria

This study was envisaged to from fresh cow dung using the Congo red staining method, and screened by measuring their endoglucanase activity (CMC enzyme activity), exoglucanase activity (Cex enzyme activity), and filter paper enzyme activity (FPA enzyme activity). Subsequently, the molecular, morphological characteristics, and growth characteristics were studied. Three strains showed clear transparent circles on the Congo red cellulose medium. LY3 exhibited strong enzyme production ability, with relatively higher enzyme activity than the other two strains. LY3 was found to be a dominant strain that required a temperature of 40℃and pH 7.0 for optimal growth. Based on the molecular and morphological characteristics, the three strains were preliminarily identified as Bacillus. Bangladesh J. Bot. 53(3): 849-856, 2024 (September) Special

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Journal
Bangladesh Journal of Botany
Published
2024-10-14
DOI
https://doi.org/10.3329/bjb.v53i30.76632
Primary Topic
Biofuel production and bioconversion
Type
article
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article

Identification and Growth Characteristics of Cellulase Producing Bacteria

Jingyi Lv, Qingrong Wang, Chao Wu
Bangladesh Journal of Botany
Biofuel production and bioconversion
article

Identification and Growth Characteristics of Cellulase Producing Bacteria

Jingyi Lv, Qingrong Wang, Chao Wu
article en

Abstract

This study was envisaged to from fresh cow dung using the Congo red staining method, and screened by measuring their endoglucanase activity (CMC enzyme activity), exoglucanase activity (Cex enzyme activity), and filter paper enzyme activity (FPA enzyme activity). Subsequently, the molecular, morphological characteristics, and growth characteristics were studied. Three strains showed clear transparent circles on the Congo red cellulose medium. LY3 exhibited strong enzyme production ability, with relatively higher enzyme activity than the other two strains. LY3 was found to be a dominant strain that required a temperature of 40℃and pH 7.0 for optimal growth. Based on the molecular and morphological characteristics, the three strains were preliminarily identified as Bacillus. Bangladesh J. Bot. 53(3): 849-856, 2024 (September) Special

Bangladesh Journal of Botany
Zunyi Normal College (CN), Tongren University (CN)
Openalex Percentile: Top 83%
Biofuel production and bioconversion
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