Second‐degree burns treatment: Preclinical in vivo approaches using natural latex rubber containing <scp><i>Aloe vera</i></scp> extract

Abstract Natural rubber latex (NRL) obtained from Hevea brasiliensis is emerging as a viable and economical alternative for biomedical treatments, particularly for dermal injuries. Its effectiveness is enhanced when combined with bioactive molecules, such as those found in Aloe vera (AV), known for their healing properties and are commonly used in low‐cost wound healing therapies. Thus, this study evaluated the physicochemical and biological properties of NRL incorporated with 25%, 35%, and 50% AV, both in vitro and in vivo. FTIR analyses revealed only physical interactions between NRL and AV, facilitating their release, as evidenced by the release profile. In vitro assays indicated improved biocompatibility in samples composed of 25% and 35% AV, while complete cell death occurred at 50% AV. Histological evaluations of second‐degree burn models in rats showed greater re‐epithelialization of the epidermis with 35% AV after 14 days of treatment. However, 50% AV highlighted the importance of avoiding overdosing to prevent tissue necrosis. Despite the differences observed between in vitro and in vivo results, NRL membranes containing AV are a promising approach for future clinical trials in skin wounds treatment, offering an effective and affordable solutions for skin regeneration in clinical settings.