Information value of latex test for pathogenic burkholderia serological diagnostics using microscopy and video digital recording system

Objective: the purpose of the work is assessment of the possibility of using instrumental assay to record the results of the latex test in the detection of pathogenic burkholderia. Monoclonal based latex agglutination test was prepared according to a previously developed technique. The latex agglutination test results were obtained using a “Nikon Eclipse TE 2000-U HMC/System microscope”, as w ell as the video-digital hardware-software com plex “Expert-lab”. The results of an objective assessment of the latex agglutination test were obtained using statistical analysis. The use of “Nikon Eclipse” microscope for visual accounting of microagglutination increased the sensitivity of the latex test to 1•10 7 CFU/ml. The “Expert-lab” make arrangements for quantitative assessment of the scanned image of the latex test results, which makes it possible to determine the threshold values for discrimination between positive and negative samples. Objective information about the presence or absence of agglutination is recorded. Numerical values of the latex test intensity between samples and controls are compared using system software. Also we can a possibility to save protocols and images of the study, to archive the obtained data. The use of “Nikon Eclipse TE 2000-U HMC/System” microscope for visual recording of the results and on the “Expert-lab” video digital system for objective evaluation of data when searching for pathogenic burkholderia allow s us to document and to archive the data obtained, to conduct in-laboratory quality control of studies and increase the sensitivity of the latex-agglutination reaction.