Effect of Moderate Beer Intake on the Lipid Composition of Human Red Blood Cell Membranes

<b>Background/Objectives</b>: Growing evidence suggests that erythrocyte membrane lipids are subject to changes during their lifespan. Factors such as the type of dietary intake and its composition contribute to the changes in red blood cell (RBC) membranes. Due to the high antioxidant content of beer, we aimed to investigate the effect of moderate beer consumption on the lipid composition of RBCs membranes from healthy overweight individuals. <b>Methods</b>: We conducted a four-weeks, prospective two-arm longitudinal crossed-over study, where participants (<i>n</i> = 36) were randomly assigned to alcohol-free beer group or traditional beer group. The lipids of RBCs membranes were assessed at the beginning and the end of the intervention by thin-layer chromatography. <b>Results</b>: Four-weeks of alcohol-free beer promoted changes in fatty acids (FA), free cholesterol (FC), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) (<i>p</i> < 0.05). Meanwhile, traditional beer intake led to changes in FA, FC, phospholipids (PL), PE and PC (<i>p</i> < 0.05). The observed alterations in membrane lipids were found to be independent of sex and BMI as influencing factors. <b>Conclusions</b>: The lipid composition of erythrocyte membranes is distinctly but mildly influenced by the consumption of both non-alcoholic and conventional beer, with no effects on RBC membrane fluidity.