Comparison of Detection Methods for Lactoferrin Iron Saturation

Due to the extensive application of lactoferrin in food, medicine, and other fields, quality control is particularly important. As a key quality indicator of lactoferrin, the accurate detection of iron saturation is crucial for ensuring the quality and efficacy of lactoferrin products. However, there is currently no standardized method for detecting iron saturation in lactoferrin within the industry, leading to variations in test results as different manufacturers may employ different detection techniques. Therefore, selecting more accurate and reliable methods for detecting iron saturation in lactoferrin is essential for quality control and the standardized production of lactoferrin products. This paper aims to provide a scientific reference for researchers in choosing the appropriate detection method for lactoferrin iron saturation. The study examines the current widely used detection methods for lactoferrin iron saturation, including saturation analysis (Method 1) and ultravioletvisible spectrophotometry (Method 2). Through in-depth analysis and comparison of the principles, accuracy, and operational complexity of these methods, the results indicate that Method 1 generally yields higher iron saturation values and demonstrates better stability and consistency in repeated measurements. This comparative study aims to offer valuable references for the precise detection of lactoferrin iron saturation, thereby promoting research and development in related fields.