RP-HPLC APPROACH FOR CONCURRENT QUANTIFICATION OF TRIFLURIDINE AND TIPIRACIL PHARMACEUTICAL AND IN BULK FORMS: DEVELOPMENT AND VALIDATION

The proposed RP-HPLC approach effectively quantifies tipiracil and trifluridine in pharmaceutical dosage forms, offering high sensitivity, linearity, and repeatability. Using a phosphate based buffer pH 4.6 and a carrier liquid of methanol with phosphate buffer solution (70:30 v/v), the method employs a Symmetry C18 column (4.6 x 150mm, 5?m, XTerra) with UV absorbance measurement at 273 nm ,the flow capacity of 1.0 ml/min and the run time was carried for 7mins. The retention duration of Tipracil is 2.003min and 5.067min for Trifluridine. The linearity range is 25-125 ?g/ml for both drugs while the regression coefficient lies at ? 0.999. The precision of developed method is confirmed by %RSD values under 2%, and recovery rates of 97-102% demonstrate accuracy and minimal excipient interference, making it suitable for routine quality control.