Establishment of heterozygous LMOD2 knockout human embryonic stem cell line (ZZUNEUi022-A-1) using CRISPR/Cas9 system
Dilated Cardiomyopathy (DCM), a prevalent form of cardiomyopathy, is characterized by ventricular dilation and systolic dysfunction. Its etiology is intricate, encompassing multiple genetic and environmental elements. The LMOD2 (Leiomodin 2) gene has been demonstrated to be closely associated with the pathogenesis of DCM. In this study, a pure cell line was generated by knocking out the LMOD2 gene, and a DCM cell model was established through induced differentiation, thus providing a powerful experimental approach for further understanding the pathogenesis of DCM. It also provides a potential research orientation for the early diagnosis and individualized treatment of DCM.
Authors
- Guangli Guo
- Yoshimichi Sai (ORCID: https://orcid.org/0000-0003-2954-1532)
- Haitao Su (ORCID: https://orcid.org/0000-0002-8388-7502)
- Hui Zhang (ORCID: https://orcid.org/0000-0002-3116-5217)
- Xiaowei Li (ORCID: https://orcid.org/0000-0002-4850-1301)
- Lihua Zhang (ORCID: https://orcid.org/0000-0003-2543-1547)
- En Li (ORCID: https://orcid.org/0000-0002-7144-961X)
- Sun Liqiang
- Chunjin Zhang (ORCID: https://orcid.org/0000-0002-6596-7373)
- Youxu Jiang
- Jing Li (ORCID: https://orcid.org/0009-0003-6354-6975)
Institutions
- Second Affiliated Hospital of Zhengzhou University (CN)
- First Affiliated Hospital of Zhengzhou University (CN)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2024-10-19
- DOI
- https://doi.org/10.1016/j.scr.2024.103586
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China