Protocol for mapping T cell activation using single-cell RNA-seq
Stimulation of CD4 T cells with anti-CD3/CD28 is a commonly used model to study T cell activation. Here, we present a protocol for investigating T cell activation based on anti-CD3/CD28 bead stimulation and single-cell RNA sequencing (scRNA-seq). We describe the workflow from the isolation of human peripheral blood mononuclear cells (PMBCs) and CD4 T cell enrichment to anti-CD3/CD28 bead stimulation, scRNA-seq, and data analysis. For complete details on the use and execution of this protocol, please refer to Li et al.<sup>1</sup>.
Authors
- Ni Hong (ORCID: https://orcid.org/0000-0002-9404-3110)
- Wenfei Jin (ORCID: https://orcid.org/0000-0002-0028-3739)
- Shiya Yu
- Yifei Liu (ORCID: https://orcid.org/0000-0002-4571-2226)
- Hui Li (ORCID: https://orcid.org/0000-0002-7516-7126)
- Xuefei Wang (ORCID: https://orcid.org/0000-0001-8218-2044)
- Junliang Wang (ORCID: https://orcid.org/0000-0003-0699-1418)
- Yue Hu
Institutions
- Southern University of Science and Technology (CN)
- Shanghai Institute of Nutrition and Health (CN)
- University of Chinese Academy of Sciences (CN)
- China Medical University (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-10-19
- DOI
- https://doi.org/10.1016/j.xpro.2024.103409
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Southern University of Science and Technology
- Special Project for Research and Development in Key areas of Guangdong Province
- Shenzhen Science and Technology Innovation Program
- Basic and Applied Basic Research Foundation of Guangdong Province