Optimization of the Production of Soluble Recombinant TEV Protease in Two E. coli Strains
The solubility of the mutant form of TEV protease increased in <i>E. coli</i> BL21 (DE3) compared to its wild form. Also, using additives such as betaine to the lysis buffer, increased the solubility of N23F in <i>E. coli</i> BL21 (DE3) and origami strains.
Authors
- Mehdi Shahriari
- Fatemeh Shafiee (ORCID: https://orcid.org/0000-0001-6484-1931)
- M. Hamid (ORCID: https://orcid.org/0000-0002-4834-4502)
- Fatemeh Moazen
Institutions
- Isfahan University of Medical Sciences (IR)
Publication Details
- Journal
- Avicenna Journal of Medical Biotechnology
- Published
- 2024-10-19
- DOI
- https://doi.org/10.18502/ajmb.v16i4.16744
- Primary Topic
- Viral Infectious Diseases and Gene Expression in Insects
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Isfahan University of Medical Sciences