Isolation and transcriptional profiling of antigen-presenting cells from mouse lung and MLNs following intranasal immunization
Dendritic cells (DCs) play a central role in the initiation of the adaptive immune response. Here, we present a protocol for isolating and transcriptionally profiling antigen-presenting cells (APCs) from the mouse lung and mediastinal lymph nodes (MLNs) following intranasal immunization. We describe steps for preparing single-cell suspensions from the lung and MLN, along with the detection and RNA sequencing (RNA-seq) of antigen-presenting DCs. This protocol offers a broadly applicable approach for identifying variations in DC subpopulations under diverse experimental conditions. For complete details on the use and execution of this protocol, please refer to Youhui et al.<sup>1</sup>.
Authors
- Shengbo Cao (ORCID: https://orcid.org/0000-0002-9787-7882)
- Youhui Si (ORCID: https://orcid.org/0000-0001-8909-221X)
- Anita S. Chong (ORCID: https://orcid.org/0000-0003-0460-0196)
- Qiang Wang (ORCID: https://orcid.org/0000-0003-3123-3889)
- Yihan Wang (ORCID: https://orcid.org/0000-0001-7440-417X)
- Xiuyu Wang (ORCID: https://orcid.org/0000-0001-6293-988X)
Institutions
- Huazhong Agricultural University (CN)
- University of Chicago (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-10-19
- DOI
- https://doi.org/10.1016/j.xpro.2024.103400
- Citations
- 1
- Primary Topic
- Immunotherapy and Immune Responses
- Type
- article
- Field-Weighted Citation Impact
- 0.24
Funders
- National Natural Science Foundation of China
- National Institutes of Health
- Fundamental Research Funds for the Central Universities
- National Institute of Allergy and Infectious Diseases
- National Institute of Biomedical Imaging and Bioengineering