Qualitative Analysis of Neomycin in Virtue of HPLC Estimation

Neomycin, an aminoglycoside antibiotic, is widely used in various pharmaceutical formulations for treating bacterial infections. Accurate determination of its concentration is critical for ensuring drug efficacy and safety. Preformulation studies play a critical role in understanding the physicochemical properties of drugs to ensure the development of stable and effective dosage forms. In this study, a comprehensive preformulation analysis of neomycin was conducted with an emphasis on its qualitative analysis and estimation through High-Performance Liquid Chromatography (HPLC). The study focuses on optimizing HPLC conditions, including mobile phase composition, flow rate, and detection wavelength, to achieve a robust analytical procedure for neomycin quantification. Standard neomycin solutions were prepared and analyzed using a reverse-phase C18 column. The method demonstrated excellent linearity over a specified concentration range, with minimal interference from other components in the formulation. Specificity, accuracy, and precision tests were conducted to validate the method, confirming its suitability for routine quality control in pharmaceutical applications. The results suggest that HPLC can provide a rapid and reliable method for neomycin estimation, aiding in both qualitative and quantitative analyses. Also, the results demonstrated the efficiency of HPLC in determining neomycin content with high sensitivity and precision. This preformulation study provides a reliable foundation for the development of neomycin-based pharmaceutical dosage forms.