SWATH‐MS Based Secretome Proteomic Analysis of <i>Pseudomonas aeruginosa</i> Against MRSA
The study uses Sequential Window Acquisition of All Theoretical Fragment Ion Mass Spectra (SWATH)-MS in conjunction with secretome proteomics to identify key proteins that Pseudomonas aeruginosa secretes against methicillin-resistant Staphylococcus aureus (MRSA). Variations in the inhibition zones indicated differences in strain resistance. Multivariate statistical methods were applied to filter the proteomic results, revealing five potential protein biomarkers, including Peptidase M23. Gene ontology (GO) analysis and sequence alignment supported their antibacterial activity. Thus, SWATH-MS provides a comprehensive understanding of the secretome of P. aeruginosa in its action against MRSA, guiding future antibacterial research.
Authors
- Han‐Ju Chien (ORCID: https://orcid.org/0000-0003-0858-4349)
- Chien‐Chen Lai (ORCID: https://orcid.org/0000-0002-7133-8266)
- Kuo‐Tung Tang (ORCID: https://orcid.org/0000-0002-5468-1329)
- Jingwen Huang
- Cheng‐Yu Kuo (ORCID: https://orcid.org/0000-0001-6211-5581)
- Yi‐Feng Zheng (ORCID: https://orcid.org/0009-0004-6925-1040)
- Nien‐Jen Hu (ORCID: https://orcid.org/0000-0002-0917-0677)
- Yu‐Sheng Lin (ORCID: https://orcid.org/0000-0002-2825-0955)
- Wei‐Chen Wang (ORCID: https://orcid.org/0009-0008-1682-9821)
- Chih‐Jui Chang
Institutions
- Asia University (TW)
- National Chung Hsing University (TW)
- National Chiayi University (TW)
- China Medical University (TW)
- Tzu Chi University (TW)
- Taichung Veterans General Hospital (TW)
- National Yang Ming Chiao Tung University (TW)
Publication Details
- Journal
- PROTEOMICS
- Published
- 2024-10-17
- DOI
- https://doi.org/10.1002/pmic.202300649
- Citations
- 1
- Primary Topic
- Bacterial Identification and Susceptibility Testing
- Type
- article
- Field-Weighted Citation Impact
- 0.38