Extraction, Purification and Identification of The Bioactive Compound Sulforaphane from Broccoli (Brassica Oleracea Var. Italica) By HPLC

The study included extraction, purification and diagnosing of the bioactive compound sulforaphane from broccoli (Brassica oleracea var. Italica) and dietary supplement of sulforaphane (Double Wood) by HPLC using different solvents including DMSO, HPLC water, acetonitrile, and dichloromethane. Sulforaphane an isothiocyanate widely distributed in Brassicaceae vegetables that comes from the enzymatic hydrolysis of glucoraphanin through myrosinase. The results of the HPLC chromatogram of the purified sulforaphane appearing 4 peaks including sulforaphane at high percentage 82.715%, HPLC chromatogram of extracted sulforaphane by HPLC water where appearing 8 peaks including sulforaphane at low percentage 4.542%, and HPLC chromatogram of the sulforaphane supplement (Double Wood) extracted by acetonitrile where appearing 4 peaks including 2 peaks of sulforaphane at percentage 5.545% and 39.038% respectively, while HPLC chromatograms of all extracts by DCM contain sulforaphane at percentage ranging from 99.904%-100%. The results of HPLC chromatogram are compared to the standard sulforaphane which diagnosed at retention time 2.640 min. and wavelength 254 nm.