Protocol for oil red O staining of low-density lipoproteins for in vivo cell treatment
Low-density lipoproteins (LDLs) are the most abundant circulating lipoproteins and the most critical factor in the development of atherosclerosis. This protocol allows the staining of LDLs with oil red O to monitor particle uptake in bright-field microscopy. Here, we describe how to stain isolated LDLs using oil red O and how to use them to monitor LDL uptake in time-lapse experiments or fixed cells.
Authors
- Valeria Pecce (ORCID: https://orcid.org/0000-0002-5693-2988)
- Simone Bini (ORCID: https://orcid.org/0000-0002-1006-4636)
- Marcello Arca (ORCID: https://orcid.org/0000-0003-3786-0883)
- Daniele Tramontano (ORCID: https://orcid.org/0000-0002-6514-2451)
- Laura D’Erasmo (ORCID: https://orcid.org/0000-0002-9174-4667)
- Ilenia Minicocci (ORCID: https://orcid.org/0000-0002-9801-4501)
- Alessia Di Costanzo
- Stella Covino
Institutions
- Sapienza University of Rome (IT)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-10-18
- DOI
- https://doi.org/10.1016/j.xpro.2024.103376
- Citations
- 1
- Primary Topic
- Lipid metabolism and disorders
- Type
- article
- Field-Weighted Citation Impact
- 0.40
Funders
- Sapienza Università di Roma