Development of a rapid LFA test based on direct RT-LAMP for diagnosis of SARS-CoV-2
By utilizing the RT-LAMP in combination with the LFA, it is possible to diagnose SARS-CoV-2 rapidly without the need for RNA extraction. The entire process from sample collection to test interpretation takes only 75-90 min, and the results can be interpreted by untrained individuals with the naked eye. By employing the ORF8 gene as a diagnostic target and eliminating the need for RNA extraction, the direct RT-LAMP-LFA method achieves a significant breakthrough that was not previously reported.
Authors
- Zohreh Hojati (ORCID: https://orcid.org/0000-0003-4831-0123)
- Bahareh Maleki (ORCID: https://orcid.org/0000-0002-3894-7246)
- William Chi Shing Cho (ORCID: https://orcid.org/0000-0003-4174-4586)
- Moein Dehbashi
- Negar Sadeghi
- Neda Shirazi
Institutions
- Isfahan University of Technology (IR)
- University of Isfahan (IR)
- Queen Elizabeth Hospital (CN)
Publication Details
- Journal
- Practical Laboratory Medicine
- Published
- 2024-10-18
- DOI
- https://doi.org/10.1016/j.plabm.2024.e00437
- Citations
- 4
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.74
Funders
- Isfahan University of Medical Sciences
- National Institute for Genetic Engineering and Biotechnology
- University of Isfahan