Insight into Chemical Composition and Anti-inflammatory Activities of Essential Oil from Flowers, Leaves and Vine Stems of Cissampelopsis volubilis Miq.

Fresh flower, leaf, and vine stem materials of Cissampelopsis volubilis Miq., collected from the wild in Lao Cai province of Vietnam, were hydrodistilled to obtain essential oils.The oil yields were 0.49%, 0.82% and 0.28% (v/w) for flowers, leaves, and vine stems, respectively, calculated on a dry weight basis.The major components of these essential oils, that were analyzed using gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS), included: -pinene (32.4%) and -pinene (39.6%) in flower oil; -pinene (55.8%) and -pinene (23.2%) in leaf oil; -pinene (65.7%) and -copaene (5.9%) in vine stem oil.The essential oil from leaves exhibited strong anti-inflammatory effect, with IC50 of 22.07 2.01 g/mL in inhibiting nitric oxide (NO) production in RAW 264.7 (ATCC -TIB-71TM) cells.This finding paves the way for further research into the potential use of this plant's leaves in treating inflammation-related diseases.To the best of our knowledge, this is the first report on the chemical composition and anti-inflammatory activity of essential oils of C. volubilis flowers, leaves, and vine stems.