Photon-counting three-dimensional fluorescence imaging based on the transport of intensity equation
A transport of intensity equation (TIE) based three-dimensional (3D) fluorescence imaging using photon-counting detection was proposed for low light-level bioimaging applications. The number of photons required to achieve the quantitative phase measurement using the proposed system was investigated experimentally and numerically. Results show that the feasibility of reconstructing phase information with an average number of photons is greater than about 9 per pixel in our plant cells.
Authors
- Inbarasan Muniraj (ORCID: https://orcid.org/0000-0002-1399-170X)
- Yasuhiro Awatsuji (ORCID: https://orcid.org/0000-0001-9967-1178)
- Manoj Kumar (ORCID: https://orcid.org/0000-0003-2870-0677)
- Naru Yoneda (ORCID: https://orcid.org/0000-0001-9964-5420)
- Shiori Matsuda (ORCID: https://orcid.org/0009-0004-9580-6783)
- Takashi Murata (ORCID: https://orcid.org/0000-0002-1543-0439)
- Osamu Matoba (ORCID: https://orcid.org/0000-0001-9767-0059)
- Marin Shoda
Institutions
- Alliance University (IN)
- Kajima Corporation (Japan) (JP)
- Kanagawa Institute of Technology (JP)
- Kyoto Institute of Technology (JP)
- University Alliance (GB)
- Kobe University (JP)
Publication Details
- Journal
- Optics Express
- Published
- 2024-10-18
- DOI
- https://doi.org/10.1364/oe.540000
- Citations
- 2
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.32
Funders
- Japan Society for the Promotion of Science
- Core Research for Evolutional Science and Technology
- Adaptable and Seamless Technology Transfer Program through Target-Driven R and D