Protocol for constructing and characterizing recombinant vectored vaccines for rabies virus
Recombinant vectored vaccines provide economical, rapidly engineered, and tailored immunization strategies. Here, we present a protocol for constructing and characterizing recombinant vectored vaccines for rabies virus (RV). We describe steps for generating replication-competent vesicular stomatitis virus (rcVSV) by replacing vesicular stomatitis virus (VSV) glycoprotein (G) with RV G gene and for recovering rcVSV by co-transfecting baby hamster kidney (BHK-21) cells with the antigenomic VSV cDNA plasmid (pVSV). We then detail procedures for the functional, structural, and molecular characterization of generated rcVSV-RV-Gs.
Authors
- Manar E. Khalifa (ORCID: https://orcid.org/0000-0002-1947-6792)
- Muhammad Munir (ORCID: https://orcid.org/0000-0003-4038-0370)
Institutions
- Lancaster University (GB)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-10-17
- DOI
- https://doi.org/10.1016/j.xpro.2024.103392
- Citations
- 2
- Primary Topic
- Rabies epidemiology and control
- Type
- article
- Field-Weighted Citation Impact
- 0.59
Funders
- Newton Fund
- Texas Biomedical Research Institute
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council