Microglia morphological response to mesenchymal stromal cell extracellular vesicles demonstrates EV therapeutic potential for modulating neuroinflammation
This study comprehensively demonstrates the effects of MSC-EVs on human microglial morphology, cytokine secretion, cellular proteome, and mitochondrial content. Our high-throughput, rapid, low-cost morphometric approach enables screening of MSC-EV batches and manufacturing conditions to enhance EV function and mitigate EV functional heterogeneity in a disease relevant manner. This approach is highly generalizable and can be further adapted and refined based on selection of the disease-relevant signal, target cell, and therapeutic product.
Authors
- Jana M. Carpenter (ORCID: https://orcid.org/0000-0002-6773-7205)
- Hannah M. Hynds (ORCID: https://orcid.org/0000-0002-5486-095X)
- Ross A. Marklein (ORCID: https://orcid.org/0000-0001-8045-0375)
- Kanupriya R. Daga
- Kelly M. Hines (ORCID: https://orcid.org/0000-0001-9125-5268)
- Levi Benjamin Wood (ORCID: https://orcid.org/0000-0002-4248-0888)
- Sara Bitarafan (ORCID: https://orcid.org/0000-0003-4584-1420)
- Andrew M. Larey
- Maria G. Morfin
- Kailin Chen
Institutions
- Georgia Institute of Technology (US)
- University of Georgia (US)
- The Wallace H. Coulter Department of Biomedical Engineering (US)
- Franklin College (US)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2024-10-17
- DOI
- https://doi.org/10.1186/s13036-024-00449-w
- Citations
- 8
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 1.87
Funders
- National Science Foundation
- National Institutes of Health