An antibody nanopore-enabled microsensor for detection of osteoprotegerin

Abstract This paper introduces an antibody-based nanopore thin film sensor for detecting osteoprotegerin (OPG) in buffer solutions and serum samples, offering significant improvements over current commercial enzyme-linked immunosorbent assay kits in terms of cost, specificity, and selectivity. Capable of detecting OPG concentrations as low as 0.25 pg ml −1 —far below the limits of traditional lab equipment—this sensor requires only about 1 μl of serum for effective measurement. The utilization of reference sensors helps reduce non-specific binding, enhancing the sensor’s accuracy. Its affordability and operational simplicity make it ideal for point-of-care monitoring of OPG levels in real-time patient care.