A DNA Origami Bubble Blower for Liposome Production

While liposomes are commonly produced on the industrial scale with diameters in the hundreds of nanometers to micrometer size range, the variation from the mean diameter can be significant. In nature, functional liposome-like systems can be as small as tens of nanometers in diameter but reproducible synthetic production at this size scale is not easily achievable. Here we outline the development of a DNA origami "bubble blower"-a nanoscale ring able to seed and constrain liposome formation. The bubble blower has the potential to be employed in a reusable fashion for production of nanometric liposomes. It improves currently available DNA origami liposome seeding techniques by expanding the range of compatible detergents and introducing solid support integration with potential for semiautomated laboratory scale production.