Ac-[K-Aib-C(3,9-Acm; 6,12-SSNC(Acm)QENSDK)]4-NH2

Advances in synthetic peptide methodologies have enabled the development of macromolecules mimicking protein properties and have found applications in various fields, particularly in immunology. Furthermore, Sequential Oligopeptide Carriers (SOCn and CPSOC) have been designed as multi-functional core molecules, to which multiple bio-cargos can be anchored, allowing the construction of high molecular weight molecules (>3000 Da) capable of inducing a strong immune response. This study presents the design and synthesis of the Ac-[K-Aib-C(3,9-Acm; 6,12-SSNC(Acm)QENSDK)]4-NH2 peptide conjugate of branched architecture. The peptide epitope S128SNCQENSDK137 belongs to the V. berus basic phospholipase A2, a member of the European viper species’ most lethal protein families. The peptide epitope was synthesized according to the SPPS Fmoc/tBu strategy and characterized by HR-ESI-MS and NMR experiments, while the conjugate was purified by RP-HPLC and characterized by HR-ESI-MS.