Natural sea water and artificial sea water are not equivalent in plastic leachate contamination studies

<ns4:p> Background Plastic contamination is one of the concerns of our age. With more than 150 million tons of plastic floating in the oceans, and a further 8 million tons arriving to the water each year, in recent times the scientific community has been studying the effects these plastics have on sea life both in the field and with experimental approaches. Laboratory based studies have been using both natural sea water and artificial sea water for testing various aspects of plastic contamination, including the study of chemicals leached from the plastic particles to the water. We set out to test this equivalence, looking at the leaching of heavy metals form plastic particles. Methods We obtained leachates of polyvinyl chloride plastic pre-production nurdles both in natural and artificial sea water and determined the elements in excess from untreated water by Inductively coupled plasma – optical emission spectrometry. We then used these different leachates to assess developmental success in the tunicate <ns4:italic>Ciona intestinalis</ns4:italic> by treating fertilised eggs through their development to hatched larvae. Results Here we report that chemical analysis of polyvinyl chloride plastic pre-production pellet leachates shows a different composition in natural and artificial sea water. We find that the zinc leaching from the plastic particles is up to five times higher in natural seawater than in artificial seawater, and this can have an effect in the toxicological studies derived. Indeed, we observe different effects in the development of <ns4:italic>C. intestinalis</ns4:italic> when using leachates in natural or artificial sea water. We also observe that not all artificial sea waters are suitable for studying the development of the tunicate <ns4:italic>C. intestinalis</ns4:italic> . Conclusions Our results show that, at least in this case, both types of water are not equivalent to produce plastic leachaetes and suggest that precaution should be taken when conclusions are derived from results obtained in artificial sea water. </ns4:p>