A novel method of Francisella infection of epithelial cells using HeLa cells expressing fc gamma receptor
In this report, we proposed a new cell-based infection system for Francisella infection using HeLa-FcγRII cells and demonstrated its effectiveness. This system has the potential to accelerate cell-based infection assays, such as large-scale genetic screening, and to provide new insights into Francisella infection in epithelial cells, which has been difficult to analyze in phagocytic cells.
Authors
- Kenta Watanabe (ORCID: https://orcid.org/0000-0002-3357-7207)
- Akihiko Uda (ORCID: https://orcid.org/0000-0003-3089-6496)
- Takashi Shimizu (ORCID: https://orcid.org/0000-0003-1294-0799)
- Masahisa Watarai (ORCID: https://orcid.org/0000-0003-3477-3135)
- Takemasa Nakamura (ORCID: https://orcid.org/0000-0002-3317-7334)
- Kohei Arasaki (ORCID: https://orcid.org/0000-0003-0647-3565)
- Ryōsuke Takahashi (ORCID: https://orcid.org/0000-0002-1407-9640)
- Naho Nishinakama
Institutions
- Tokyo University of Pharmacy and Life Sciences (JP)
- Yamaguchi University (JP)
- National Institute of Infectious Diseases (JP)
Publication Details
- Journal
- BMC Infectious Diseases
- Published
- 2024-10-17
- DOI
- https://doi.org/10.1186/s12879-024-10083-y
- Citations
- 2
- Primary Topic
- Bacillus and Francisella bacterial research
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Japan Society for the Promotion of Science