Hepatitis Delta Antigen Retains the Assembly Domain as the Only Rigid Entity
The hepatitis delta virus (HDV) S-HDAg and L-HDAg antigens are the two isoforms of the single protein encoded by the viral genome. Together with the double-stranded RNA genome they form the HDV ribonucleoprotein (RNP) complex. In the context of a divide-and-conquer approach, we used a combination of cell-free protein synthesis and proton (<sup>1</sup>H)-detected fast magic angle spinning solid-state NMR at highest magnetic field to characterize S-HDAg. We sequentially assigned <i>denovo</i> its isolated N-terminal assembly domain using less than 1 mg of fully protonated protein. Our results show that the assembly domain is the sole rigid component in S-HDAg, with its structure remaining fully conserved within the full-length protein. In contrast, the rest of the protein remains dynamic. This work provides the necessary foundation for future studies of the viral RNP.
Authors
- Lauriane Lecoq (ORCID: https://orcid.org/0000-0002-5995-3733)
- Anja Böckmann (ORCID: https://orcid.org/0000-0001-8149-7941)
- Morgane Callon (ORCID: https://orcid.org/0000-0002-0618-2293)
- Alexander A. Malär (ORCID: https://orcid.org/0000-0003-1026-9583)
- Marie‐Laure Fogeron (ORCID: https://orcid.org/0000-0002-2146-8740)
- Beat H. Meier (ORCID: https://orcid.org/0000-0002-9107-4464)
- Alexander B. Barnes (ORCID: https://orcid.org/0000-0003-3748-8508)
- Yang Yang (ORCID: https://orcid.org/0000-0002-6489-4459)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- ETH Zurich (CH)
Publication Details
- Journal
- Journal of the American Chemical Society
- Published
- 2024-10-16
- DOI
- https://doi.org/10.1021/jacs.4c09409
- Citations
- 3
- Primary Topic
- Hepatitis B Virus Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.16
Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Agence Nationale de Recherches sur le Sida et les Hépatites Virales
- China Scholarship Council