Quantitative Analysis of Cell-Free RNA at Attomolar Level Using CRISPR/Cas Digital Imaging Platform
Quantitative analysis of cell-free RNA (cfRNA) in plasma sample can be used for screening, diagnosing, and prognosticating of multiple diseases. Here, we report a quantitative CRISPR/Cas digital imaging platform (qCasdip) for the detection of various cfRNAs, including circular RNAs and miRNAs, in clinical samples at the attomolar (aM) level without the need for preamplification. Digital counting strategy provides qCasdip quantitative ability with a linear detection range of 10<sup>2</sup>-10<sup>6</sup> aM. Meanwhile, qCasdip demonstrated cfRNA profiling in clinical plasma samples, improving the diagnosis of breast cancer. These data highlight the potential of qCasdip to quantitatively assess the molecular patterns of specific cfRNA panels in plasma, thereby providing a novel liquid biopsy solution to enhance disease diagnosis.
Authors
- Yongyuan Zhang (ORCID: https://orcid.org/0000-0001-9337-1553)
- Yonghua Wu (ORCID: https://orcid.org/0009-0001-2146-765X)
- Kaixiang Zhang (ORCID: https://orcid.org/0000-0002-6812-6342)
- Yanan Li (ORCID: https://orcid.org/0000-0002-9528-0273)
- Yige Wu
- Fenglei Quan
- Yan Liang (ORCID: https://orcid.org/0000-0002-3002-5431)
- Junli Zhang (ORCID: https://orcid.org/0000-0001-6083-5112)
- Hua Gao (ORCID: https://orcid.org/0000-0002-9698-5830)
- Ru Xu (ORCID: https://orcid.org/0000-0002-4508-0855)
Institutions
- Zhengzhou University (CN)
- Advanced Pharma (US)
- Respiratory and Meningeal Pathogens Research Unit (ZA)
- Beijing Academy of Science and Technology (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-10-16
- DOI
- https://doi.org/10.1021/acs.analchem.4c04067
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- National Natural Science Foundation of China