Protocol to measure cell avidity between cord blood-derived NK cells and leukemia cell line KG-1a

The avidity between immune cells and target cells is crucial for effective immune responses. Here, we present a protocol to measure single-cell avidity between cord blood-derived natural killer (NK) cells and the human acute myelocytic leukemia cell line KG-1a. We describe the steps for isolating cord blood mononuclear cells, purifying NK cells, and culturing KG-1a cells. We then detail the procedures for detecting cell avidity between NK cells and KG-1a cells using the acoustic force-based z-Movi cell avidity analyzer. For complete details on the use and execution of this protocol, please refer to Wang et al.¹.