Plant plasmodesmata bridges form through ER-dependent incomplete cytokinesis
Diverging from conventional cell division models, plant cells undergo incomplete division to generate plasmodesmata communication bridges between daughter cells. Although fundamental for plant multicellularity, the molecular events leading to bridge stabilization, as opposed to severing, remain unknown. Using electron tomography, we mapped the transition from cell plate fenestrae to plasmodesmata. We show that the endoplasmic reticulum (ER) connects daughter cells across fenestrae, and as the cell plate matures, fenestrae contract, causing the plasma membrane (PM) to mold around constricted ER tubes. The ER's presence prevents fenestrae fusion, forming plasmodesmata, whereas its absence results in closure. The ER-PM protein tethers MCTP3, MCTP4, and MCTP6 further stabilize nascent plasmodesmata during fenestrae contraction. Genetic deletion in <i>Arabidopsis</i> reduces plasmodesmata formation. Our findings reveal how plants undergo incomplete division to promote intercellular communication.
Authors
- Clément Chambaud (ORCID: https://orcid.org/0000-0002-0351-212X)
- Mélina Petrel (ORCID: https://orcid.org/0000-0002-2564-4350)
- Ziqiang Patrick Li (ORCID: https://orcid.org/0000-0002-3148-1711)
- Antoine Taly (ORCID: https://orcid.org/0000-0001-5109-0091)
- Marie‐Cécile Caillaud (ORCID: https://orcid.org/0000-0002-0348-7024)
- Emmanuelle Bayer (ORCID: https://orcid.org/0000-0001-8642-5293)
- Yvon Jaillais (ORCID: https://orcid.org/0000-0003-4923-883X)
- Lysiane Brocard (ORCID: https://orcid.org/0000-0002-9397-1660)
- Fanny Decoeur (ORCID: https://orcid.org/0000-0002-0155-9119)
- Andrea Paterlini (ORCID: https://orcid.org/0000-0002-1777-3160)
- William A. Prinz (ORCID: https://orcid.org/0000-0002-9053-2398)
- Étienne Gontier (ORCID: https://orcid.org/0000-0002-9604-9065)
- Felix Campelo (ORCID: https://orcid.org/0000-0002-0786-9548)
- Lucie Hoornaert
- Hortense Moreau (ORCID: https://orcid.org/0000-0001-9739-3987)
- Tatiana de Souza Moraes (ORCID: https://orcid.org/0000-0002-0146-6860)
- Marie Glavier (ORCID: https://orcid.org/0000-0002-7100-7045)
- Magali Grison (ORCID: https://orcid.org/0000-0002-3080-3686)
- Marija Smokvarska (ORCID: https://orcid.org/0000-0002-7256-0803)
- Jessica Pérez‐Sancho (ORCID: https://orcid.org/0000-0002-6201-3240)
- Jules D. Petit (ORCID: https://orcid.org/0000-0003-4652-2843)
- Amit S. Joshi (ORCID: https://orcid.org/0000-0003-3321-0744)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- École Normale Supérieure de Lyon (FR)
- Centre National de la Recherche Scientifique (FR)
- Université de Bordeaux (FR)
- Inserm (FR)
- Institute of Photonic Sciences (ES)
- Université Paris Cité (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Laboratoire de Biochimie Théorique (FR)
- Southwestern Medical Center (US)
- Bordeaux Population Health (FR)
- Laboratoire de Biogenèse Membranaire (FR)
- Laboratoire Reproduction et Développement des Plantes (FR)
- University of Tennessee at Knoxville (US)
- The University of Texas Southwestern Medical Center (US)
Publication Details
- Journal
- Science
- Published
- 2024-10-31
- DOI
- https://doi.org/10.1126/science.adn4630
- Citations
- 18
- Primary Topic
- Plant nutrient uptake and metabolism
- Type
- article
- Field-Weighted Citation Impact
- 10.79