ENDOTHELIN-1 IN ADRENAL CORTICOCYTES WITH A COMBINATION OF CRANIO BRAIN INJURY AND ENDOTOXEMIA

Neuroinflammation in traumatic brain injury (TBI) and endotoxemia caused by bacterial lipopolysaccharide have an indirect effect through blood vessels on paracrine and autocrine processes regulating steroidogenesis of adrenal corticocytes. The vasoconstrictor peptide endothelin-1 plays a key role in blood vessel homeostasis. This protein, as a regulator, may be involved in the integration of the listed mechanisms of action on the adrenal glands during endotoxemia and inflammation. Purpose of the study: immunohistochemical characterization of the localization sites and content of endothelin-1 in adrenal corticocytes during a combination of TBI and endotoxemia in the experiment. Materials and methods. Moderate TBI was reproduced in mature rats using a falling weight model weighing 200 g, endotoxemia was induced by intraperitoneal administration of pyrogenal at a total dose of 100 μg/kg body weight. Endothelin-1 was detected in adrenal tissues using the immunohistochemical method and morphometric analysis of the images was performed. Results. In all experimental groups, the stability of the morphometric characteristics of the ratio of the calving cortex and the adrenal medulla was found. With the combination of TBI and endotoxemia, there is a decrease in the area of the nucleus and cytoplasm of adrenal corticocytes and, as a consequence, the nuclear-cytoplasmic ratio. A morphological study revealed an increase in the content of endothelin-1 in the cells of the adrenal cortex, while in the adrenocytes of the medulla, a significant positive reaction to the presence of the peptide was not detected. Conclusion. The results obtained demonstrate that the combination of TBI and endotoxemia causes dysfunction of adrenal cortex cells with an increase in the synthesis of endothelin-1 in them.