Evaluating tomato lines resistance to Late Blight and molecular genetic screening with the use of molecular markers

Relevance. Tomato late blight caused by Phytophthora infestans can cause almost 100% yield loss in open ground in cool and humid conditions. At the same time, the genetic characteristics of P. infestans allow it to overcome the genetic resistance of host plants over time, which requires breeders to look for new genes for resistance to late blight and to obtain new varieties that have several resistance genes at once.Material and methods. 12 tomato lines, or a total of 335 plants, were obtained from the N.N. Timofeev breeding station collection and planted in an artificially infected background. For molecular genetic screening, 12 plants from the Kr6 line were used. The markers linked to the late blight resistance genes Ph-3, R1, and R3a were utilized.Results. On an artificial infectious background, phenotypic evaluation of tomato resistance to late blight showed 1 line of plants free of pathogen damage, 5 lines of plants fully afflicted by late blight, and 6 lines with only partial plant damage. According to molecular genetic investigation the resistant plants were heterozygotes for the Ph-3 gene. Furthermore, the R1 gene was present in most of the plants under study; but, without the Ph-3 gene, this gene did not provide plant resistance against late blight.Conclusions. The results of this research led to the selection of tomato plants for further breeding that were resistant to late blight. It was shown that markers linked to the Ph-3 and R1 genes might be used for marker-mediated selection. Furthermore, it has been established that tomato plants are more effectively protected against P. infestans when several resistance genes are present.