Metagenomic Study Reveals Phage–Bacterial Interactome Dynamics in Gut and Oral Microbiota in Pancreatic Diseases
Individuals with pancreatic-related health conditions usually show lower diversity and different composition of bacterial and viral species between the gut and oral microbiomes compared to healthy individuals. We performed a thorough microbiome analysis, using deep shotgun sequencing of stool and saliva samples obtained from patients with chronic pancreatitis (CP), pancreatic ductal adenocarcinoma (PDAC), and healthy controls (HCs).We observed similar microbiota composition at the species level in both the gut and oral samples in PDAC patients compared to HCs, among which the most distinctive finding was that the abundance of oral-originated <i>Fusobacterium nucleatum</i> species did not differ between the oral and the gut samples. Moreover, comparing PDAC patients with HCs, <i>Klebsiella oxytoca</i> was significantly more abundant in the stool samples of PDAC patients, while <i>Streptococcus</i> spp. showed higher abundance in both the oral and stool samples of PDAC patients. Finally, the most important finding was the distinctive gut phage-bacterial interactome pattern among PDAC patients. CrAssphages, particularly <i>Blohavirus</i>, showed mutual exclusion with <i>K. oxytoca</i> species, while <i>Burzaovirus</i> showed co-occurrence with <i>Enterobacteriaceae</i> spp., which have been shown to be capable of inducing DNA damage in human pancreatic cells ex vivo. The interactome findings warrant further mechanistic studies, as our findings may provide new insights into developing microbiota-based diagnostic and therapeutic methods for pancreatic diseases.
Authors
- M. Morell (ORCID: https://orcid.org/0000-0002-1708-0110)
- Esther Fort (ORCID: https://orcid.org/0000-0002-9584-1210)
- Xavier Aldeguer (ORCID: https://orcid.org/0000-0003-4597-8339)
- Anna Feliu (ORCID: https://orcid.org/0000-0003-2074-2231)
- Berta Laquente (ORCID: https://orcid.org/0000-0002-2477-1673)
- Montserrat Capdevila (ORCID: https://orcid.org/0000-0003-0649-9773)
- Ville N. Pimenoff (ORCID: https://orcid.org/0000-0002-0813-7031)
- Vı́ctor Moreno (ORCID: https://orcid.org/0000-0002-2818-5487)
- A. García (ORCID: https://orcid.org/0000-0001-8388-0522)
- Laura Pardo (ORCID: https://orcid.org/0000-0003-1515-6748)
- Marc Llirós (ORCID: https://orcid.org/0000-0001-8456-4230)
- Maria T. Albiol-Quer
- Eric J. Duell (ORCID: https://orcid.org/0000-0001-5256-0163)
- Robert Carreras‐Torres (ORCID: https://orcid.org/0000-0002-2925-734X)
- Anna Bahí (ORCID: https://orcid.org/0000-0001-9774-9218)
- L. J. Garcia‐Gil (ORCID: https://orcid.org/0000-0002-5064-8361)
- Laura Vilà-Quintana (ORCID: https://orcid.org/0000-0001-7495-0031)
- Débora Pozas
- María Ortiz (ORCID: https://orcid.org/0000-0002-8973-0493)
Institutions
- Universitat de Vic - Universitat Central de Catalunya (ES)
- Karolinska Institutet (SE)
- Institut d'Investigació Biomédica de Bellvitge (ES)
- Institut Català d'Oncologia (ES)
- Institut d'Investigació Biomèdica de Girona (ES)
- Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (ES)
- Hospital Universitari de Girona Doctor Josep Trueta (ES)
- Centro de Investigación Biomédica en Red de Cáncer (ES)
- Universitat de Barcelona (ES)
- University of Oulu (FI)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2024-10-12
- DOI
- https://doi.org/10.3390/ijms252010988
- Citations
- 5
- Primary Topic
- Pancreatic and Hepatic Oncology Research
- Type
- article
- Field-Weighted Citation Impact
- 1.41
Funders
- European Molecular Biology Organization
- Fundació la Marató de TV3
- Turun Yliopisto
- Åbo Akademi
- Biocenter Finland