Snorkel‐tag based affinity chromatography for recombinant extracellular vesicle purification
Extracellular vesicles (EVs) are lipid nanoparticles and play an important role in cell-cell communications, making them potential therapeutic agents and allowing to engineer for targeted drug delivery. The expanding applications of EVs in next generation medicine is still limited by existing tools for scaling standardized EV production, single EV tracing and analytics, and thus provide only a snapshot of tissue-specific EV cargo information. Here, we present the Snorkel-tag, for which we have genetically fused the EV surface marker protein CD81, to a series of tags with an additional transmembrane domain to be displayed on the EV surface, resembling a snorkel. This system enables the affinity purification of EVs from complex matrices in a non-destructive form while maintaining EV characteristics in terms of surface protein profiles, associated miRNA patterns and uptake into a model cell line. Therefore, we consider the Snorkel-tag to be a widely applicable tool in EV research, allowing for efficient preparation of EV standards and reference materials, or dissecting EVs with different surface markers when fusing to other tetraspanins in vitro or in vivo.
Authors
- Markus Schosserer (ORCID: https://orcid.org/0000-0003-2025-0739)
- Jaroslaw Jacak (ORCID: https://orcid.org/0000-0002-4989-1276)
- André Görgens (ORCID: https://orcid.org/0000-0001-9198-0857)
- Matthias Hackl (ORCID: https://orcid.org/0000-0002-4136-7293)
- Sylvia Weilner
- Marianne Pultar
- Regina Grillari‐Voglauer (ORCID: https://orcid.org/0000-0002-5487-2838)
- Samir Barbaria
- Samir EL Andaloussi (ORCID: https://orcid.org/0000-0003-4468-9113)
- Giulia Corso (ORCID: https://orcid.org/0000-0002-1145-0862)
- Jørgen Kjems (ORCID: https://orcid.org/0000-0003-4128-9317)
- Yan Yan (ORCID: https://orcid.org/0000-0002-5993-0750)
- Dhanu Gupta (ORCID: https://orcid.org/0000-0003-0163-2678)
- Madhusudhan Reddy Bobbili (ORCID: https://orcid.org/0000-0001-9955-0675)
- Stefan Vogt (ORCID: https://orcid.org/0000-0002-8034-5513)
- Carolina Patrioli
Institutions
- Institute of Molecular Biotechnology (AT)
- Karolinska University Hospital (SE)
- Aarhus University (DK)
- Karolinska Institutet (SE)
- University of Oxford (GB)
- Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA (AT)
- Evercyte (Austria) (AT)
- University of Applied Sciences Upper Austria (AT)
- Austrian Cluster for Tissue Regeneration (AT)
- Omiics (Denmark) (DK)
- Ludwig Boltzmann Institute for Digital Health and Prevention (AT)
- University of Duisburg-Essen (DE)
- Medical University of Vienna (AT)
Publication Details
- Journal
- Journal of Extracellular Vesicles
- Published
- 2024-10-01
- DOI
- https://doi.org/10.1002/jev2.12523
- Citations
- 13
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 3.04
Funders
- Austrian Science Fund