The Impact of Combination Ag: Se Core: Shell Nanoparticles Synthesized by Plasma Jet with Plantago lanceolata on REF Cell Line

Abstract The process well-thought-out for isolating new and novel ant-microbial components is though natural sources which is considered the best option. Plantago lanceolata is a medicinal plant that holds several bioactive compounds, containing tannins, phenolic compound, flavonoids and alkaloids. These benefits contain potential anti-inflammatory, antioxidant, antimicrobial. This study among to synthesize Ag: Se NPs at (125 and 250) μg mL −1 concentrations in ratio (0:10, 1:9, 2:8, 3:7) respectively. Characterization of the produced silver nanoparticles involved UV-visible spectroscopy, X-ray diffraction (XRD), and field emission scanning electron microscopy (FESEM). Cytotoxicity against normal cells line (REF) were assessed using several diluted concentrations, Ag: Se NPs (25, 50, and 100%) and concentrations of P. lanceolata (125 and 250) μg mL −1 . The optimal XRD pattern and UV-visible were attained at a ratio (3:7). At a concentration of 100% Ag: Se NPs at 250 μg mL-1, the most significant cytotoxic on REF cell lines was stated. However, the addition of combined. Ag: Se NPs (125 and 250) μg mL −1 with P. lanceolata (125 and 250) μg mL −1 resulted in reduced cytotoxicity, indicating no synergistic effect at all. In conclusion, this study successfully synthesized Ag: Se NPs and investigating their combined effects with P. lanceolata on REF cell lines.