A Very Simple and Feasible Colorimetric-Electrochemical Bimodal Method for H₂O₂ Detection in Human Serum

Hydrogen peroxide (H 2 O 2 ) possesses disinfectant, antiseptic, deodorizing, and cleansing properties. It is commonly used to clean wounds, ulcers, abscessed sinuses, and pus in the ear. Herein, a colorimetric-electrochemical bimodal method for the detection of hydrogen peroxide in serum was constructed, when using a platinum sheet electrode electrocatalyzes H 2 O 2 producing hydroxyl radical, resulting in oxidising TMB and giving a color and current change. After optimaztion, cyclic voltammetry, differential pulse voltammetry and ultraviolet spectrophotometry constituting a colorimetric-electrochemical bimodal method were used to establish four linear relationships within the concentration range of 10.00–1000.00 μM. The limit of detection was found to be 3.33 μM. Additionally, the method’s recoveries ranged from 98.88% to 101.10% with a relative standard deviation of no more than 3.70% when using the standard addition method in serum, which indicates its potential for practical medical testing.