Precise Control of Intracellular Trafficking and Receptor‐Mediated Endocytosis in Living Cells and Behaving Animals
Intracellular trafficking, an extremely complex network, dynamically orchestrates nearly all cellular activities. A versatile method that enables the manipulation of target transport pathways with high spatiotemporal accuracy in vitro and in vivo is required to study how this network coordinates its functions. Here, a new method called RIVET (Rapid Immobilization of target Vesicles on Engaged Tracks) is presented. Utilizing inducible dimerization between target vesicles and selective cytoskeletons, RIVET can spatiotemporally halt numerous intracellular trafficking pathways within seconds in a reversible manner. Its highly specific perturbations allow for the real-time dissection of the dynamic relationships among different trafficking pathways. Moreover, RIVET is capable of inhibiting receptor-mediated endocytosis. This versatile system can be applied from the cellular level to whole organisms. RIVET opens up new avenues for studying intracellular trafficking under various physiological and pathological conditions and offers potential strategies for treating trafficking-related disorders.
Authors
- Ching‐Lin Kao
- Pin‐Chiao Sun
- Wan-Yu Chung
- Binxing Wu
- Lily Wang (ORCID: https://orcid.org/0000-0003-0659-0139)
- Chun‐Hao Chen (ORCID: https://orcid.org/0000-0001-7887-235X)
- Jinjun Yang (ORCID: https://orcid.org/0000-0002-9939-151X)
- Yi‐Chien Chuang (ORCID: https://orcid.org/0000-0002-4545-2321)
- Chia-Yu Shih (ORCID: https://orcid.org/0000-0003-2044-113X)
- Tzu‐Ying Lin (ORCID: https://orcid.org/0000-0002-3428-9944)
- Oliver I. Wagner (ORCID: https://orcid.org/0000-0001-6322-1853)
- Yi‐Ching Wang (ORCID: https://orcid.org/0000-0002-7694-2067)
- Hsuan Cheng (ORCID: https://orcid.org/0000-0003-4895-3815)
- Yu‐Chun Lin (ORCID: https://orcid.org/0000-0002-9629-7560)
- Shiau‐Chi Chen
- Yi‐Ling Tai
- Hsien‐Chu Wang (ORCID: https://orcid.org/0009-0009-3623-5550)
- Ningxiang Zeng (ORCID: https://orcid.org/0009-0002-5744-449X)
- Po‐Han Chiang (ORCID: https://orcid.org/0000-0003-4100-4051)
- Yu‐Ting Chiu (ORCID: https://orcid.org/0000-0002-5964-1270)
- Grace Y. Liu (ORCID: https://orcid.org/0000-0001-6138-2434)
- Chiao‐Yun Chen (ORCID: https://orcid.org/0000-0003-4113-2547)
- Yin Fang (ORCID: https://orcid.org/0009-0009-7888-3679)
- Odvogmed Bayansan
Institutions
- National Yang Ming Chiao Tung University (TW)
- National Taiwan University (TW)
- National Tsing Hua University (TW)
- National Cheng Kung University (TW)
Publication Details
- Journal
- Advanced Science
- Published
- 2024-10-14
- DOI
- https://doi.org/10.1002/advs.202405568
- Citations
- 3
- Primary Topic
- Cellular transport and secretion
- Type
- article
- Field-Weighted Citation Impact
- 0.98
Funders
- Academia Sinica
- National Health Research Institutes
- National Tsing Hua University
- National Science and Technology Council