Synthesis, Characterization and Influence of Copper Nanoparticles on Growth of Vigna mungo L. Hepper CO-7 Variety

The copper nanoparticle was synthesized by employing Cnidoscolus aconitifolius (Mill) leaf extract. The nanoparticles were characterized by UV, FTIR and SEM studies. The SEM image showed a spherical structure. The bio-stimulant effect of green synthesized copper nanoparticles on Vigna mungo L. Hepper was investigated. Seed germination, biochemical, antioxidant, and enzyme activities of the copper nanoparticles were studied. The germination and morphological characteristics were superior at 100 mg/L copper nanoparticle-treated seeds. The biochemical parameters such as chlorophyll (0.701±0.107 mg/g), carbohydrate (4.657±0.090 mg/g), protein (4.396±0.335 mg/g), amino acid (18.557±0.638 mg/g), phenol (10.824±0.53 mg/g), flavonoid (3.644±0.171 mg/g), alkaloid (6.529±0.45 mg/g), and tannin (12.159±0.218 mg/g) were higher at a nanoparticle concentration of 100 mg/L. As far as the enzymatic activities, the maximum activity of nitrate reductase (4.505±0.203 mg/g) and α-amylase (13.195±0.285 mg/g) were observed with 100 mg/L copper nanoparticle-treated seedlings. The in vitro antioxidant activities were also studied.