Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins

Channelrhodopsins (ChRs) are light-gated ion channels widely used to optically activate or silence selected electrogenic cells, such as individual brain neurons. Here, we describe identifying and characterizing a set of anion-conducting ChRs (ACRs) from diverse taxa and representing various branches of the ChR phylogenetic tree. The Mantoniella squamata ACR (MsACR1) showed high sensitivity to yellow-green light ( λ max at 555 nm) and was further engineered for optogenetic applications. A single amino-acid substitution that mimicked red-light-sensitive rhodopsins like Chrimson shifted the photosensitivity 20 nm toward red light and accelerated photocurrent kinetics. Hence, it was named red and accelerated ACR, raACR. Both wild-type and mutant are capable optical silencers at low light intensities in mouse neurons in vitro and in vivo , while raACR offers a higher temporal resolution.

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Publication Details

Journal
eLife
Published
2024-10-14
DOI
https://doi.org/10.7554/elife.90100.3
Citations
1
Primary Topic
Photoreceptor and optogenetics research
Type
article
Field-Weighted Citation Impact
0.27

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article

Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins

Thomaz Mansini Carrenho Fabrin, Johannes Oppermann, Peter Hegemann, Andrey Rozenberg et al.
1 citations
eLife
Photoreceptor and optogenetics research
0.27
article

Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins

Thomaz Mansini Carrenho Fabrin, Johannes Oppermann, Peter Hegemann, Andrey Rozenberg, Matthias Prigge, Cristian González‐Cabrera, Oded Béjà, Rafael ES Parker
article en
1 citations

Abstract

Channelrhodopsins (ChRs) are light-gated ion channels widely used to optically activate or silence selected electrogenic cells, such as individual brain neurons. Here, we describe identifying and characterizing a set of anion-conducting ChRs (ACRs) from diverse taxa and representing various branches of the ChR phylogenetic tree. The Mantoniella squamata ACR (MsACR1) showed high sensitivity to yellow-green light ( λ max at 555 nm) and was further engineered for optogenetic applications. A single amino-acid substitution that mimicked red-light-sensitive rhodopsins like Chrimson shifted the photosensitivity 20 nm toward red light and accelerated photocurrent kinetics. Hence, it was named red and accelerated ACR, raACR. Both wild-type and mutant are capable optical silencers at low light intensities in mouse neurons in vitro and in vivo , while raACR offers a higher temporal resolution.

eLifeVol. 12
Technion – Israel Institute of Technology (IL), Humboldt-Universität zu Berlin (DE), Center for Behavioral Brain Sciences (DE), Research Network (United States) (US), Leibniz Institute for Neurobiology (DE)
Alexander von Humboldt-Stiftung, EU Joint Programme – Neurodegenerative Disease Research, Deutsche Forschungsgemeinschaft, Israel Science Foundation
Life in Land
Openalex Percentile: Top 43%
Photoreceptor and optogenetics research
0.27
DOIOpen Access PDFOpenAlex
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