Proteus mirabilis—Mediated Green Synthesis of Magnesium Nanoparticles: Optimization and Their Antimicrobial Activity

The present study was carried out to produce magnesium nanoparticles (MgNPs) through biological method by using microbes which is not only sustainable but economical too. After careful screening, Proteus mirabilis BTCB21 (MN956898) was selected for the synthesis of magnesium nanoparticles. Various parameters like temperature, pH and substrate concentration were adjusted to optimize magnesium nanoparticles and investigate their effect on reaction time and size of nanoparticles. Characterization of magnesium nanoparticles was completed by UV-Vis spectrophotometer which exhibited peak at 350 nm, Fourier-transform infrared (FTIR) exposed presence of various groups like N–H, C≡C, C–Cl, C–Br, C–F and O–H. Scanning Electron Microscopy (SEM) displayed irregular nanoparticles with average size of 463.06 nm. The results revealed optimization conditions for magnesium nanoparticles to be at 37°C temperature, pH 7 and 1 g of substrate concentration was selected for optimization. Antibacterial activity was also carried out; highly resistant pathogenic bacteria Staphylococcus aureus BTCB02 and Salmonella typhi BTCB06 were selected for antibacterial activity. The zones of inhibition ranged between 11 to 27 mm respectively. Magnesium nanoparticles prepared through biological method has great potential for applications in different fields especially biomedical; to treat antibiotic resistant illnesses.