Characterization of Bacillus pacificus G124 and Its Promoting Role in Plant Growth and Drought Tolerance

Drought represents a major environmental threat to global agricultural productivity. Employing plant growth-promoting rhizobacteria (PGPR) offers a promising strategy to enhance plant growth and resilience under drought stress. In this study, the strain G124, isolated from the arid region of Qinghai, was characterized at the molecular level, and its ability to enhance plant drought tolerance was validated through pot experiments. The findings revealed that the strain G124 belongs to <i>Bacillus pacificus</i>, with a 99.93% sequence similarity with <i>B. pacificus</i> EB422 and clustered within the same clade. Further analysis indicated that the strain G124 demonstrated a variety of growth-promoting characteristics, including siderophore production, phosphate solubilization, and the synthesis of indole-3-acetic acid (IAA), among others. Moreover, inoculation with <i>B. pacificus</i> G124 resulted in significant enhancements in plant height, leaf area, chlorophyll content, relative water content, and root development in both <i>Arabidopsis thaliana</i> and <i>Medicago sativa</i> seedlings under drought conditions. Additionally, G124 boosted antioxidant enzyme activities and osmolyte accumulation, while reducing malondialdehyde (MDA) and reactive oxygen species (ROS) levels in <i>M. sativa</i> seedlings exposed to drought. These findings suggest that <i>B. pacificus</i> G124 holds significant promise for enhancing plant drought tolerance and could be effectively utilized in crop management strategies under arid conditions.