Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights
This study examines a case involving a 7-year-old child who developed dengue symptoms following Qdenga vaccination. Despite initial negative diagnostic results, molecular analysis confirmed an infection with DENV4. Next-generation sequencing detected viral RNA from both DENV2 and DENV4 serotypes, which were identified as vaccine-derived strains using specific primers. Phylogenetic analysis further confirmed that these sequences belonged to the Qdenga vaccine rather than circulating wild-type viruses. This case underscores the critical need for precise diagnostic interpretation in vaccinated individuals to avoid misdiagnosis and to strengthen public health surveillance. A comprehensive understanding of vaccine-induced viremia is essential for refining dengue surveillance, improving diagnostic accuracy, and informing public health strategies in endemic regions.
Authors
- Felipe Campos de Melo Iani (ORCID: https://orcid.org/0000-0002-3629-8529)
- Keldenn Melo Farias Moreno (ORCID: https://orcid.org/0000-0002-0424-9212)
- Luíz Marcelo Ribeiro Tomé (ORCID: https://orcid.org/0000-0002-1477-5558)
- Paulo Eduardo de Souza da Silva
- Ana Cândida Araújo e Silva (ORCID: https://orcid.org/0000-0002-7852-6809)
- Mauricio Teixeira Lima (ORCID: https://orcid.org/0000-0002-0830-3909)
- Sílvia Helena Sousa Pietra Pedroso (ORCID: https://orcid.org/0000-0002-1177-7435)
- Talita Adelino (ORCID: https://orcid.org/0000-0002-1471-0084)
- Luíz Carlos Júnior Alcântara (ORCID: https://orcid.org/0000-0002-6769-9931)
- Marta Giovanetti (ORCID: https://orcid.org/0000-0002-5849-7326)
- Vagner Fonseca (ORCID: https://orcid.org/0000-0001-5521-6448)
- Natália Rocha Guimarães (ORCID: https://orcid.org/0000-0002-9859-5895)
- Náthale Rodrigues Pinheiro (ORCID: https://orcid.org/0000-0003-4521-9121)
- Carolina Senra Alves de Souza
Institutions
- Universidade Federal de Minas Gerais (BR)
- Universidade do Estado da Bahia (BR)
- Università Campus Bio-Medico (IT)
- Stellenbosch University (ZA)
- Ezequiel Dias Foundation (BR)
- Universidade Federal dos Vales do Jequitinhonha e Mucuri (BR)
- Secretaria da Saúde (BR)
- Fundação Oswaldo Cruz (BR)
Publication Details
- Journal
- Viruses
- Published
- 2024-10-12
- DOI
- https://doi.org/10.3390/v16101603
- Citations
- 4
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 2.17