Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients
We confirm that stimulating 4 × 10<sup>5</sup> PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses.
Authors
- Hongjiao Chen
- Li Wang (ORCID: https://orcid.org/0000-0002-9270-4544)
- Yuanqi Yang (ORCID: https://orcid.org/0009-0005-7571-4539)
- Ying Huang (ORCID: https://orcid.org/0000-0002-4627-1661)
- Weixian Chen (ORCID: https://orcid.org/0000-0001-6973-9338)
- Di Mu (ORCID: https://orcid.org/0000-0002-7211-941X)
Institutions
- Dalian Medical University (CN)
- Second Affiliated Hospital of Chongqing Medical University (CN)
- Guizhou Provincial People's Hospital (CN)
- Chongqing Medical University (CN)
Publication Details
- Journal
- BMC Biotechnology
- Published
- 2024-10-14
- DOI
- https://doi.org/10.1186/s12896-024-00908-8
- Primary Topic
- Hepatitis B Virus Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Chongqing Medical University