Relationship between polymorphism of GDF5 and CAPN1 genes in kalmyk breed cattle with economic and biological traits

The article presents the results of polymorphism of the GDF5 and CAPN1 genes in cattle of the Kalmyk breed, owned by the agricultural production company “Plodovitoe” of the Maloderbetovsky district of the Republic of Kalmykia, in the amount of 60 heads. DNA samples were isolated from whole blood taken from the jugular vein by an automatic extraction method. A real-time polymerase chain reaction was carried out to determine the polymorphism T586C of the GDF5 gene and C316G of the CAPN1 gene. In the studied herd of cattle, animals with the TT genotype predominate - 68.3 % of the population, the number of animals with the TC and CC genotypes is 21.7 % and 10 %. When studying the polymorphism of the CAPN1 gene, it was revealed that 23.3 % of the bulls of the studied population of Kalmyk cattle have the desired genotype CC, while the heterozygous genotype is present in 16.7 %, and the recessive genotype GG is present in 60 % of animals. The frequency of occurrence of the T allele of the GDF5 gene is 0.792, which exceeds the indicator of the C allele (0.208) by 58.2 %, the observed frequency of TT and CC genotypes exceeds the expected indicator by 5.6 and 5.7 %, respectively, while the indicator of the TC genotype is lower than the expected frequency by 11.2 %. The frequency of occurrence of the G allele (0.683) is 36.6 % higher than that of the C allele (0.317) of the CAPN1 gene. It is noted that the GG genotype with an index of 0.600 occurs with the highest frequency, while the CG genotype has a frequency of 0.167 (43.3 % less), the CC genotype – 0.233 (36.7 % less). The indicators of the observed frequency of the CC and GG genotypes are 13.3% higher than expected, the heterozygous genotype has an excess of the expected frequency by 26.6 %. Bulls with the TT genotype of the GDF5 gene outperformed their peers with the TC and CC genotypes in all measurements. When studying the live weight of Kalmyk cattle at the age of 15 months, a positive effect of the CC genotype of the CAPN1 gene was revealed. Thus, the group of animals with this genotype had the largest live weight and surpassed their peers with the genotypes CG (by 9.9 %) and GG (by 11.1 %).