Enhancing felid conservation: Exploring the impact of in vitro culture media on domestic cat blastocyst production
This study investigated the optimization of assisted reproductive techniques for wild felid conservation, focusing on in vitro procedures using the domestic cat as a model species. The research evaluated the impact of three different in vitro culture media on blastocyst formation. Oocytes and spermatozoa were collected and processed, followed by in vitro fertilization and culture. Results returned a similar blastocyst rate (ANOVA, p > .05), over 16% across all groups. While demonstrating the potential of these techniques, further investigations are warranted to evaluate embryo quality to refine optimal protocols and their applicability in felid conservation efforts.
Authors
- María Jesús Sánchez‐Calabuig (ORCID: https://orcid.org/0000-0002-4280-992X)
- Ana Sánchez-Rodríguez (ORCID: https://orcid.org/0000-0002-3724-9470)
- Eduardo R. S. Roldán (ORCID: https://orcid.org/0000-0002-7545-4248)
- Dimitrios V. Rizos (ORCID: https://orcid.org/0000-0001-6813-3940)
- Ana Munoz‐Maceda (ORCID: https://orcid.org/0000-0001-6140-9238)
- C. Nuñez-Puente
- Joaquín Cerdeira‐Lozano (ORCID: https://orcid.org/0000-0001-8710-6454)
- Andrea Priego-González (ORCID: https://orcid.org/0009-0005-9537-6055)
Institutions
- Universidad Complutense de Madrid (ES)
- Institute of Agrifood Research and Technology (ES)
- Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (ES)
- Museo Nacional de Ciencias Naturales (ES)
Publication Details
- Journal
- Reproduction in Domestic Animals
- Published
- 2024-10-01
- DOI
- https://doi.org/10.1111/rda.14645
- Citations
- 4
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 2.17
Funders
- Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
- Comunidad de Madrid