A new hormonal protocol supports early development of in vitro‐produced embryos after transfer to anoestrus mares

The present study aimed to evaluate whether primed anoestrus mares are suitable recipients for embryos produced by intracytoplasmic sperm injection (ICSI). Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest, oral altrenogest was administered at 0.088 mg/kg and embryos (1 to 5 embryos per mare; 15 in total) were transferred 3.5 days after progesterone onset. Uterine lavage was conducted 48 h after transfer. The results revealed an 80% embryo recovery rate, and among the retrieved embryos, 67% showed evident intrauterine development. Hence, ICSI-derived embryos can be successfully transferred to primed anoestrus mares, but more studies are required to ensure further embryo development and foaling.

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Publication Details

Journal
Reproduction in Domestic Animals
Published
2024-10-01
DOI
https://doi.org/10.1111/rda.14622
Primary Topic
Reproductive Biology and Fertility
Type
article
Field-Weighted Citation Impact
0.00

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article

A new hormonal protocol supports early development of in vitro‐produced embryos after transfer to anoestrus mares

María Jesús Sánchez‐Calabuig, Lauro González‐Fernández, Pablo Fernández‐Hernández, Raúl Fernández‐González et al.
Reproduction in Domestic Animals
Reproductive Biology and Fertility
article

A new hormonal protocol supports early development of in vitro‐produced embryos after transfer to anoestrus mares

María Jesús Sánchez‐Calabuig, Lauro González‐Fernández, Pablo Fernández‐Hernández, Raúl Fernández‐González, Beatriz Macías‐García, Alfonso Gutiérrez‐Adán, Marcos Luis‐Calero, Carmen Cristina Muñoz-García, Juan Cuervo‐Arango, Alba Pizarro‐Hernández
article en

Abstract

The present study aimed to evaluate whether primed anoestrus mares are suitable recipients for embryos produced by intracytoplasmic sperm injection (ICSI). Anoestrus was confirmed in four mares and daily doses of oestradiol benzoate (6 mg in total) over 5 days were administered; after 3 days of rest, oral altrenogest was administered at 0.088 mg/kg and embryos (1 to 5 embryos per mare; 15 in total) were transferred 3.5 days after progesterone onset. Uterine lavage was conducted 48 h after transfer. The results revealed an 80% embryo recovery rate, and among the retrieved embryos, 67% showed evident intrauterine development. Hence, ICSI-derived embryos can be successfully transferred to primed anoestrus mares, but more studies are required to ensure further embryo development and foaling.

Reproduction in Domestic AnimalsVol. 59(S3)
Universidad Complutense de Madrid (ES), Universidad Cardenal Herrera CEU (ES), Universidad de Extremadura (ES)
Banco Santander, Junta de Extremadura, Universidad de Extremadura, European Regional Development Fund, Agencia Estatal de Investigación
Life below water
Openalex Percentile: Top 73%
Reproductive Biology and Fertility
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