Aerosol immunization with influenza matrix, nucleoprotein, or both prevents lung disease in pig

Current influenza vaccines are strain-specific and require frequent updates to combat new strains, making a broadly protective influenza vaccine (BPIV) highly desirable. A promising strategy is to induce T-cell responses against internal proteins conserved across influenza strains. In this study, pH1N1 pre-exposed pigs were immunized by aerosol using viral vectored vaccines (ChAdOx2 and MVA) expressing matrix (M1) and nucleoprotein (NP). Following H3N2 challenge, all immunizations (M1, NP or NPM1) reduced lung pathology, but M1 alone offered the greatest protection. NP or NPM1 immunization induced both T-cell and antibody responses. M1 immunization generated no detectable antibodies but elicited M1-specific T-cell responses, suggesting T cell-mediated protection. Additionally, a single aerosol immunization with the ChAdOx vaccine encoding M1, NP and neuraminidase reduced lung pathology. These findings provide insights into BPIV development using a relevant large natural host, the pig.

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Publication Details

Journal
npj Vaccines
Published
2024-10-13
DOI
https://doi.org/10.1038/s41541-024-00989-8
Citations
2
Primary Topic
Influenza Virus Research Studies
Type
article
Field-Weighted Citation Impact
0.77

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article

Aerosol immunization with influenza matrix, nucleoprotein, or both prevents lung disease in pig

Basudev Paudyal, Ellen C. Moorhouse, Eleni Vatzia, Susan Morris et al.
2 citations
npj Vaccines
Influenza Virus Research Studies
0.77
article

Aerosol immunization with influenza matrix, nucleoprotein, or both prevents lung disease in pig

Basudev Paudyal, Ellen C. Moorhouse, Eleni Vatzia, Susan Morris, Marta Ulaszewska, Francisco J. Salguero, Ronan MacLoughlin, Simon Gubbins, Sarah C. Gilbert, Elma Tchilian, B. Veronica Carr, Bárbara Dema, Ehsan Sedaghat-Rostami, Bhawna Sharma
article en
2 citations

Abstract

Current influenza vaccines are strain-specific and require frequent updates to combat new strains, making a broadly protective influenza vaccine (BPIV) highly desirable. A promising strategy is to induce T-cell responses against internal proteins conserved across influenza strains. In this study, pH1N1 pre-exposed pigs were immunized by aerosol using viral vectored vaccines (ChAdOx2 and MVA) expressing matrix (M1) and nucleoprotein (NP). Following H3N2 challenge, all immunizations (M1, NP or NPM1) reduced lung pathology, but M1 alone offered the greatest protection. NP or NPM1 immunization induced both T-cell and antibody responses. M1 immunization generated no detectable antibodies but elicited M1-specific T-cell responses, suggesting T cell-mediated protection. Additionally, a single aerosol immunization with the ChAdOx vaccine encoding M1, NP and neuraminidase reduced lung pathology. These findings provide insights into BPIV development using a relevant large natural host, the pig.

npj VaccinesVol. 9(1)
The Pirbright Institute (GB), University of Oxford (GB), Aerogen (Ireland) (IE)
Bill and Melinda Gates Foundation, UK Research and Innovation, Department for Environment, Food and Rural Affairs, UK Government, University of Oxford, Directorate for Biological Sciences, Animal and Plant Health Inspection Service, Medical Research Council, Biotechnology and Biological Sciences Research Council
Good health and well-being
Openalex Percentile: Top 25%
Influenza Virus Research Studies
0.77
DOIOpen Access PDFOpenAlex
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