<i>Entamoeba muris</i> mitigates metabolic consequences of high-fat diet in mice

Metabolic syndrome (MetS) is a cluster of several human conditions including abdominal obesity, hypertension, dyslipidemia, and hyperglycemia, all of which are risk factors of type 2 diabetes, cardiovascular disease, and metabolic dysfunction-associated steatotic liver disease (MASLD). Dietary pattern is a well-recognized MetS risk factor, but additional changes related to the modern Western life-style may also contribute to MetS. Here we hypothesize that the disappearance of amoebas in the gut plays a role in the emergence of MetS in association with dietary changes. Four groups of C57B/6J mice fed with a high-fat diet (HFD) or a normal diet (ND) were colonized or not with <i>Entamoeba muris</i>, a commensal amoeba. Seventy days after inoculation, cecal microbiota, and bile acid compositions were analyzed by high-throughput sequencing of 16S rDNA and mass spectrometry, respectively. Cytokine concentrations were measured in the gut, liver, and mesenteric fat looking for low-grade inflammation. The impact of HFD on liver metabolic dysfunction was explored by Oil Red O staining, triglycerides, cholesterol concentrations, and the expression of genes involved in β-oxidation and lipogenesis. Colonization with E. muris had a beneficial impact, with a reduction in dysbiosis, lower levels of fecal secondary bile acids, and an improvement in hepatic steatosis, arguing for a protective role of commensal amoebas in MetS and more specifically HFD-associated MASLD.

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Publication Details

Journal
Gut Microbes
Published
2024-10-13
DOI
https://doi.org/10.1080/19490976.2024.2409210
Citations
3
Primary Topic
Pancreatitis Pathology and Treatment
Type
article
Field-Weighted Citation Impact
1.72

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article

Entamoeba muris mitigates metabolic consequences of high-fat diet in mice

Sylvana Rozes, Seiki Kobayashi, Sandrine Adiba, Émilie Viennois et al.
3 citations
Gut Microbes
Pancreatitis Pathology and Treatment
1.72
article

Entamoeba muris mitigates metabolic consequences of high-fat diet in mice

Sylvana Rozes, Seiki Kobayashi, Sandrine Adiba, Émilie Viennois, Anne Dumay, Catherine Postic, Valérie Paradis, Maude Le Gall, Jean‐Pierre Hugot, Dominique Rainteau, Alexis Mosca, Maite Casado‐Bedmar, Éric Ogier‐Denis, Ulrich Meinzer, Maryline Roy
article en
3 citations

Abstract

Metabolic syndrome (MetS) is a cluster of several human conditions including abdominal obesity, hypertension, dyslipidemia, and hyperglycemia, all of which are risk factors of type 2 diabetes, cardiovascular disease, and metabolic dysfunction-associated steatotic liver disease (MASLD). Dietary pattern is a well-recognized MetS risk factor, but additional changes related to the modern Western life-style may also contribute to MetS. Here we hypothesize that the disappearance of amoebas in the gut plays a role in the emergence of MetS in association with dietary changes. Four groups of C57B/6J mice fed with a high-fat diet (HFD) or a normal diet (ND) were colonized or not with <i>Entamoeba muris</i>, a commensal amoeba. Seventy days after inoculation, cecal microbiota, and bile acid compositions were analyzed by high-throughput sequencing of 16S rDNA and mass spectrometry, respectively. Cytokine concentrations were measured in the gut, liver, and mesenteric fat looking for low-grade inflammation. The impact of HFD on liver metabolic dysfunction was explored by Oil Red O staining, triglycerides, cholesterol concentrations, and the expression of genes involved in β-oxidation and lipogenesis. Colonization with E. muris had a beneficial impact, with a reduction in dysbiosis, lower levels of fecal secondary bile acids, and an improvement in hepatic steatosis, arguing for a protective role of commensal amoebas in MetS and more specifically HFD-associated MASLD.

Gut MicrobesVol. 16(1)
Centre National de la Recherche Scientifique (FR), Inserm (FR), Université Paris Cité (FR), Université Paris Sciences et Lettres (FR), Hôpital Beaujon (FR), École Normale Supérieure - PSL (FR), Sorbonne Université (FR), National Institute of Infectious Diseases (JP), Assistance Publique – Hôpitaux de Paris (FR), Hôpital Saint-Antoine (FR), Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine (FR), Hôpital Robert-Debré (FR), Institut de Biologie de l'École Normale Supérieure (FR), Centre de Recherche sur l'Inflammation (FR), Centre de Recherche Saint-Antoine (FR), Institut Cochin (FR)
Laboratoire d'Excellence Inflamex, Agence Nationale de la Recherche, Institut National de la Recherche Agronomique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Zero hunger
Openalex Percentile: Top 15%
Pancreatitis Pathology and Treatment
1.72
DOIOpen Access PDFOpenAlex
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