Assessment of chemical composition of blackberries and mulberries from Covilhã region, Portugal

Berries have gained popularity recently due to the health benefits associated with their bioactive compounds. This work aimed to evaluate the physicochemical characteristics, colour, phytochemicals content and mineral content of three common berries, two types of blackberries ( Rubus fruticosus and R. ulmifolius ) and one mulberry ( Morus nigra ), collected in the Covilhã region, Portugal. R. fruticosus presented the greatest length, weight, moisture, firmness and darker colour, while R. ulmifolius showed the greatest width. On the other hand, M. nigra showed the highest ash content (2.49±0.40%). A total of 20 phenolics were identified and quantified by chromatographic techniques. Using ICP-MS and FAAS, 13 essential and 14 non-essential elements were quantified. Additionally, 54 volatiles were detected through HS-SPME/GC-MS, several compounds were herein reported for the first time in these berries. Our results show that these berries contain a high concentration of high-value bioactive compounds, significantly influencing their nutritional value, aroma and appeal to consumers, providing data to make it possible to choose more attractive and superior blackberry and mulberry varieties that meet consumer demands. • First comparative study regarding two species of Rubus spp. and one of Morus sp. • Physicochemical composition of Rubus spp. and one of Morus sp. • First phenolic and volatile profile of Portuguese blackberries and mulberries • Essential and non-essential elements in Portuguese blackberries and mulberries • Portuguese blackberries and mulberries were rich in Ca and Mg