Calotropis Procera Triggered Biochemical and Molecular Alterations In Breast Cancer Caused By DMBA In Female Albino Rats

Objectives: To investigate the in vivo anticancer effect of Calotropis procera on breast cancer induced in female albino rats with 7,12-dimethyl benz(a) anthracene (DMBA). Materials and study design: Fresh leaves of C. procera were obtained from 20 plant specimens collected from various regions in the Dawadmi governorate, Riyadh province, KSA (latitude 24.75387 and longitude 46.773686) in August 2022. Three extracts were obtained from C. procera leaves (aqueous, chloroform, and ethanolic extracts). were investigated for their anticancer activity on female albino rats that were classified into five equal groups (n=30): group 1 (control), group 2 (DMBA control), group 3 (DMBA + aqueous extract), group 4 (DMBA + chloroform extract), and group 5 (DMBA + ethanolic extract). Results: The animals in groups 3, 4, and 5 treated with C. procera leaf extract exhibited a significant decrease in body weight compared to the animals in group 2 who were only given DMBA, and the survival analysis demonstrated a highly significant disparity between the groups, with the DMBA group displaying a greater incidence of tumors in comparison to the groups treated with C. procera. Biochemical findings indicated a notable variation in the average levels of cholesterol, TG, HDL and LDL between the groups. Conclusion The results imply that aqueous, chloroform, and ethanolic C. procera leaf extracts may have preventive properties against tumor initiation and promotion.