Bacteriological and Molecular Identification of Mycoplasma Bovis and Mycoplasma bovigenitalium Isolated from Cattle and Buffaloes

This study was intended to detect the most common Mycoplasma species involved in respiratory diseases in both cattle and buffaloes. In this study, examination of 300 cattle and 200 buffaloes was carried out and then the collected samples were subjected to isolation of Mycoplasmaspecies followed by PCR confirmation. The results showed that the percent of mollicutes isolation from pneumonic lung tissues of cattle& buffaloes was 22% and 17%, respectively. Moreover, the percent of mollicutes isolation from nasal swabs of diseased cattle & buffaloes was 18% and 20%, respectively. PCR was effective in the detection of both M. bovis and M. bovigenitalium through successful amplification of the Mbo and Mbg gene at 360 and 312 bp, respectively. The minimum inhibitory concentration was performed on two field strains of M. bovis against seven different antimicrobial agents. The first strain was sensitive to Draxxin® 10 %, Marbocyle® 10%, Lincospectin®, Duocycline ® 20%, and Tilmicosin® 30%. The second strain was sensitive to Duocycline® 20% and Tilmicosin® 30% and resistant to other antimicrobial agents. Regarding the associated risk factors with Mycoplasmosis; the season was significantly correlated and the infection was 6.5 times more prevalent in the winter season than summer season. As well as the sex revealed a significant effect and 5.08 times in males more than females. Additionally, cattle were more susceptible than buffaloes for the disease prevalence. Meanwhile, the age was not a significant risk for the disease prevalence.